ionic liquid-based single drop as a simple and efficient microextraction method for simultaneous determination of aminophenol isomers in human urine, hair dye and water samples using hplc

a user-friendly and inexpensive ionic liquid-based single-drop microextraction (il-sdme) method was developed to preconcentrate trace amounts of aminophenol isomers (aps) from human urine, hair dye and water samples prior to analysis by high performance liquid chromatography-ultraviolet spectrophotometry detection (hplc-uv). under optimum conditions (i.e., 3.5 µl of 1-hexyl-3-methylimidazolium hexafluorophosphate [c6mim][pf6], 10 ml of sample, nacl free adjusted to ph 5.5, 15 min extraction time and 800 rpm agitation speed at room temperature) enrichment factors and limits of detection were ranged from 348 to 364 and 0.06 to 0.14 depending on the target analytes. the method gave good levels of repeatability with relative standard deviations varying between 3.6 and 4.2 % (n = 5). recoveries of the analytes were ranged between 87.3 and 99.3 %, showing that the matrix had a negligible effect upon extraction. finally, the proposed method was successfully applied to the analysis of different real samples such as human urine, hair dye and water samples.